Version-1.27.0
Release Date : February 2026
Major Features
Projection Traces - GMM Fitting Support
Gaussian Mixture Model (GMM) Fitting: Revolutionary new fitting approach for complex peak patterns
GMMParameterEditorDialog: Interactive parameter editor with real-time previewSupport for multiple peaks with common sigma (shared width)
Per-parameter bounds management for precise control
Fixed parameter support to constrain specific values
Peak and sigma tolerance settings for robust fitting
Transactional rollback capability for safe parameter exploration
Enhanced Peak Management:
Peak mirroring functionality based on cache status
EditPeakDetailsDialog: Edit individual peak parameters with tolerance controlPeak labeling visualization for better identification
Peak bounds synchronization across the fitting process
Automatic peak position recalculation to prevent drift
Improved Box Processing:
Three-stage processing pipeline (histogram → peak detection → fitting)
Box-centric configuration format using JSON
ProcessingBoxandProcessingStatedataclasses for structured data managementImage-level cache utilization for faster reprocessing
Hull range management with auto-zoom functionality
Overlay dragging and edge manipulation for interactive adjustments
Manual Calibration Enhancements
Advanced Optimization Methods:
ManualCalibrationDialog: Comprehensive calibration interfaceDifferential evolution for global optimization (escapes local minima)
MAD-based (Median Absolute Deviation) outlier rejection for robust fitting
Multi-start optimization for improved convergence
Objective-based refinement with configurable parameters
Calibration Workflow Improvements:
Point refinement for sub-pixel accuracy
CSV export of optimization history for analysis
Enhanced results display with detailed metrics
Architecture Refactoring
New Base Classes
BaseGUI: Minimal foundation class for all GUI componentsStandard image tab creation using template method pattern
Floating widget positioning
Unified initialization flow
Status bar integration via navigator attribute
ImageNavigatorWidget: Integrated image viewing and file management componentCombines ImageViewerWidget with FileManager for unified file browsing
Image display with tools and interactions
Navigation controls (next/prev image, next/prev file)
File path display with auto-update
Background directory scanning with progress tracking
Scan completion tracking for accurate file counts
ProcessingWorkspace: Complete workspace for image processing modules (QF, PT, EQ)Uses ImageNavigatorWidget for integrated image viewing and file management
Display panel integration with collapsible settings
Calibration settings management (center, rotation, blank/mask)
Settings persistence to JSON files
Delayed notifications for improved UI responsiveness
Data Management
ImageDataContainer: Structured image data managementCenter and rotation handling
Quadrant folding state
Blank/mask image references
Factory method for consistent creation
Quadrant Folding Improvements
Terminology Update
“Blank Image” → “Empty Cell Image”: More accurate terminology throughout the application
Enhanced UI Components
ImageSettingsPanel: Centralized settings managementCenter and rotation settings delegation
Coordinate handling improvements
Settings persistence across images
BlankMaskSettingsWidget: Dedicated widget for empty cell and mask configurationImproved Dialogs:
SetCentDialog: Now usesImageViewerWidgetfor better image displaySetAngleDialog: Integrated intoImageSettingsPanelSupport for negative rotation angles
Center and Rotation Management
Configuration Fingerprinting: Automatic cache validation
Cache invalidation when settings change
Preserved manual settings during cache operations
“Quick Center and Rotation Angle” tool for simultaneous setting
Mode Indicators: Visual feedback for center and rotation modes
Auto vs. manual mode display
Apply current center/rotation to subsequent images
Restore auto center/rotation with granular control (current image or all)
Collapsible UI
CollapsibleGroupBoxandCollapsibleRightPanelfor better space utilizationStreamlined quadrant options integration
Optimized result tab layout
X-Ray Viewer Enhancements
Slice Box Functionality: Enhanced slice visualization
Refactored Layout: Improved status bar handling
Intensity Range Handling: Better control over display ranges
Fitting Options Tab: Enabled after histogram update
Performance & Multiprocessing
Parallel Processing
EquatorWindow Multiprocessing: Significant speed improvements
ProcessPoolExecutorfor concurrent image processingWorker initialization with proper context (macOS compatibility)
Task cancellation support with real-time feedback
Stop process dialog with progress indication
Batch completion metrics and timing
Memory Management:
Automatic cleanup after batch processing
Release of large image data after UI updates
Optional memory usage monitoring
Better resource management
File Manager Enhancements
Cached Directory Scanning: Disk caching for faster loading
Multiprocessing for I/O-bound tasks
Two-phase loading (synchronous + background)
Provisional file selection during scanning
Asynchronous directory scanning with status updates
HDF5 Support Improvements:
Full HDF5 file processing across all GUIs
Frame count tracking instead of loading all images
Master file preference when both master and data files exist
Progress tracking for HDF5 processing
Proper handling of HDF5 frame ranges
UI/UX Improvements
Display Enhancements
DisplayOptionsPanel: Centralized display controlsIntensity value validation to prevent errors
Persist intensity values across sessions
Brightness, contrast, and zoom controls
ImageViewerWidget: Enhanced image displayIntegrated tool support
Built-in zoom handling (mouse wheel, zoom rectangle)
Panning support
Axis management improvements
Zoom Functionality
DoubleZoomWidget: Advanced dual-view zoomDynamic crop radius adjustment
Improved intensity normalization
Red dot cursor tracking fix
Subpixel-level accuracy for calibration
Status and Progress
HDF5 processing progress display in all GUIs
Enhanced progress bar formatting with detailed status
File indices in status bar
Scan completion indicators
Confirmation dialog when closing with running tasks
Bug Fixes
Data Processing
Fixed pandas.errors.InvalidIndexError by removing duplicate “Meridian Sigma” column
Fixed center coordinate handling in multiple GUIs
Fixed blank/mask handling in QuadrantFolder
Clipped image values to [0, 255] range to prevent underflow/overflow
Fixed pixel threshold check for invalid pixels (≤ threshold)
Fixed zoom rectangle removal in ProjectionBoxTab
UI Issues
Fixed double zoom red dot not following mouse
Fixed zoom functionality in SetCentDialog
Fixed center display updates in QuadrantFoldingGUI and ImageSettingsPanel
Fixed background processing in QuadrantFoldingGUI
Fixed progress bar calculation in multiple GUIs
Fixed file manager name errors
Fixed notification handling when opening images
Processing Issues
Fixed rotation handling to use original coordinates
Fixed image size consistency after rotation
Fixed cache handling for quadrant folded images
Fixed geometric center display for quadrant folded images
Removed axis inversion issues
Code Quality & Architecture
Refactoring
Component Architecture: New modular GUI architecture with clear separation of concerns
BaseGUI provides unified initialization framework
ImageNavigatorWidget handles file management and navigation
ProcessingWorkspace integrates image processing workflows
Reorganized file structure following Python conventions (
ui_widgets→ui/widgets)Removed obsolete methods and unused attributes
Streamlined blank image and mask handling
Better separation of concerns across modules
Removed redundant center-related code
Deprecated unused methods (getRotatedImage, centerizeImage, movePeaks)
State Management
Configuration fingerprinting for cache validation
Improved state management in QuadrantFolder
Better handling of manual vs automatic settings
Cache persistence for processing state
Transactional updates with rollback support
Code Cleanup
Removed debug print statements
Removed commented-out code
Standardized naming conventions
Improved error handling and logging
Better resource cleanup
Development & Build
Documentation
Added comprehensive architecture SVG diagrams (v1.25-1.27)
GitHub Actions Build & Release Workflow documentation
Manual calibration circle-band objective documentation
Performance optimization and parallel processing guides
Build guides for Linux DEB packages
Build System
GitHub Actions Workflow: Automated multi-platform builds
Linux, macOS, and Windows support
Multi-architecture builds
Artifact management and release creation
DMG creation for macOS with quarantine attribute removal
Debian package handling
Packaging Updates:
Inno Setup installer script for Windows
Advanced Installer project (MuscleX-1.26.0.aip)
Windows icon support (AppIcon.ico)
Updated spec files with sklearn data and PySide6 imports
Source distribution creation in workflow
Development Tools
VSCode launch configurations for debugging
Multiple GUI modes
Headless mode support
Various functionality testing configs
Updated
.gitignore:venv, .idea directories
temp_files, docs/_build
test_guide directory
test.log files
Testing
New Test Cases
PT_FittingGaussians_Horizontal: GMM fitting validationEIGER_PT_Convex_Hull_Vertical: EIGER detector supportMAR_PT_Convex_Hull_Vertical: MAR detector convex hull testsUpdated test suite for new box configuration format
Headless test enhancements for background processing
Test Infrastructure
Updated environment tester with latest package versions
Improved test result validation
Better test image organization
Enhanced test utilities
Known Issues & Future Work
Additional documentation needed for GMM fitting workflows
Continue performance optimization for very large HDF5 files
Further UI refinements based on user feedback
Extended testing on additional detector types
.. note:: Version 1.27.0 is a full release. This version has been tested on 3.10 on Ubuntu 22.04
Total Changes: 346+ commits since v1.26.1
Key Contributors: Development team continues to enhance MuscleX with focus on advanced fitting algorithms, performance optimization, and improved user experience.